Figure 6
From: A simple method using CRISPR-Cas9 to knock-out genes in murine cancerous cell lines
Evaluation of SUCCESS to target Antxr2 in murine ovarian cancer cell line ID8-luc2. The schema of knockout of Antxr2 gene by SUCCESS is shown. Agarose gel images show the genotyping results. The table indicates the genotyping results of 93 clones. 5′, 5′ site between the genomic region and the cassette; 3′, 3′ site between the genomic region and the cassette; WT, wild-type allele; −/−, homozygously knockout clones: −/−*, homozygously knockout clones without the detection of 5′ or 3′ sites ligation between the genomic region and the cassette; %KI, percentage of knock-in in 93 clones.
