Figure 3
From: Further evidence supporting a potential role for ADH1B in obesity
ADH1B regulates FABP4 expression during adipocyte differentiation. (A) Immunoblot analysis of cell lysates from adipocytes following differentiation and transduction with lentiviruses. Pre-adipocytes were transduced with lentivirus containing empty vector (Control) or vector encoding myc-DDK-tagged human ADH1B on Day 0 and Day 10 of differentiation in triplicate. Untreated pre-adipocytes were differentiated in duplicate and utilized as an additional control. Total protein was collected following complete differentiation, resolved by SDS-PAGE, and immunoblotted with antibodies specific for ADH1B, FABP4, and β-actin control. (B) Quantitative analysis of FABP4 expression in cells overexpressing ADH1B compared to empty vector control. Biological replicates were processed on the same Western blot. Fluorescence intensity was quantified and normalized to β-Actin control. Data is presented as the mean ± s.e.m. AU, Arbitrary Units; ***P = 0.0005.
