Figure 4

bpV(HOpic) alleviates IR-induced oxidative stress in cells by stregthening the antioxidant defense mechanism. Cell were pretreated with 100 nmol/L bpV(Hopic) 1 h before 4 Gy IR exposure (A) Analysis of the effect of bpV(Hopic) on total ROS production in irradiated NIH-3T3 by CM-H2DCFDA at indicated time points using flow cytometer. Bar charts represented as fold change in mean fluorescence intensity (MFI) with respective control. (B) Effect of bpV(Hopic) on Mitochondrial ROS production in irradiated NIH-3T3 by Mitosox Red at indicated time points using flow cytometer. Bar charts represented as fold change in mean fluorescence intensity (MFI) with respective control. (C) Effect of bpV(HOpic) on the levels of non-enzymatic antioxidant in irradiated NIH-3T3 cells at indicated time intervals. (D) After treatment, NIH-3T3 cells whole cell lysates were subjected to immunoblotting and densitometry analysis for indicated proteins of antioxidant defense mechanism. (E) Effect of bpV(HOpic) on the catalase activity of irradiated NIH-3T3 cells at indicated time points. (F) Effect of bpV(HOpic) on the SOD activity of irradiated NIH-3T3 cells at indicated time points. (G) Effect of bpV(HOpic) on the protein oxidation of irradiated NIH-3T3 cells assessed by nmoles of carbonyl content per mg protein at indicated time intervals. (H) Effect of bpV(HOpic) on the membrane lipid oxidation of irradiated NIH-3T3 cells assessed by nmoles of MDA content per mg protein at indicated time intervals. Error bars are mean ± SD (n = 4) from two independent experiments. *p < 0.05; **p < 0.01.