Figure 6

bpV(HOpic) protects cells against IR through AKT-induced enhanced glycolysis. (A) Effect of bpV(HOpic) on the levels of key glycolysis proteins in irradiated NIH-3T3 cells. Inset bar charts represent densitometry analysis of proteins normalized to Actin control. (B) Effect of bpV(HOpic) on the mitochondrial localization of Hexokinase II in NIH-3T3 cells at 24-h post IR exposure. (C) Glucose uptake in bpV(HOpic) pretreated 4 Gy irradiated NIH-3T3 assessed at indicated timepoints with 2-NBDG and flow cytometry. Control and bpV(HOpic) groups at 0 h represent the status of glucose uptake at the time of irradiation, represented as the mean fluorescent intensity of 2-NBDG uptake. (D) Effect of bpV(HOpic) pretreatment on the cellular ATP content of irradiated NIH-3T3 cells. Error bars are mean ± SD (n = 4) from two independent experiments. *p < 0.05; **p < 0.01; ns = non-significant.