Figure 1
From: Apelin promotes blood and lymph vessel formation and the growth of melanoma lung metastasis
Apelin overexpression enhances the migratory activity and invasive capacity of melanoma cell lines. (A) Overexpression of apelin in B16 and A375 melanoma cells increased 2D motility independently of cell density (10/30/90 cell/mm2) and surface (TC plastic vs. collagen I). Columns show the average cell speed in two experiments with three cell densities at 48 h + /− SEM. (B) Representative curves of time-dependent average speed demonstrating increased cell motility in apelin-overexpressing A375 cells regardless of the surface (non-precoated vs. collagen I coated) at 90 cell/mm2 cell density. (C–F) Representative images and quantification of tumor cell invasion. Apelin overexpression significantly increased the perimeter/area ratio of spheroids compared to spheroids formed by cells transfected with control vector. Columns, mean for three experiments; bars, standard error of the mean. *p < 0.05.
