Figure 3

Immunohistochemical analyses of the dentate gyrus 21 days after CO exposure, and the number of SOX2⁻/GFAP⁺, CD11b⁺, and NG2⁺ cells in the dentate gyrus. (A) Immunohistochemical analyses were performed using antibodies against SOX2 (red) and GFAP (green) for controls (Aa–c) at 21 days. (B) Immunohistochemical analyses were performed using antibodies against SOX2 (red) and GFAP (green) for the CO rats (Ba–c) at 21 days after CO exposure. (C) The number of SOX2⁻/GFAP⁺ cells is not significantly different between CO rats and controls. (D) Immunohistochemical analyses were performed using antibodies against CD11b (red) for controls at 21 days. (E) Immunohistochemical analyses were performed using antibodies against CD11b (red) for the CO rats at 21 days after CO exposure. (F) The number of CD11b⁺ cells is significantly lower in the CO rats 21 days after CO exposure than in the controls. (G) Immunohistochemical analyses were performed using antibodies against NG2 (green) for controls at 21 days. (H) Immunohistochemical analyses were performed using antibodies against NG2 (green) for the CO rats at 21 days after CO exposure. Nuclear staining was performed using DAPI solution. (I) The number of NG2⁺ cells is significantly lower in the CO rats 21 days after CO exposure than in the controls. Scale bars = 100 µm (Aa–c, Ba–c, D, E, G, H). Values are expressed as mean ± SEM. *p < 0.05.