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Figure 1

From: Human induced pluripotent stem cell derived hepatocytes provide insights on parenteral nutrition associated cholestasis in the immature liver

Figure 1

Transcriptome differences in human developing liver, iHLC, and adult liver. (a) Principle component analysis (PCA) plot showing variance in gene expression among iHLC from 6 independent differentiations (yellow), 4 human fetal/infant liver samples (blue), and adult liver (red). The top 2 components (PC1 and PC2) account for 99% of the variance among samples, and the variance captured within each coordinate is shown in parentheses. (b) Immunocytochemistry of fully differentiated iHLC showing cytoplasmic presence of alpha-fetoprotein (AFP). Scale bar: 25 μm. (c) Venn diagram summarizing the overlap of differentially expressed genes (DEG) that are upregulated and downregulated between developing liver vs adult liver compared with DEG in iHLC vs adult liver. DEG were genes with fold change ≥ 4 and adjusted p-value < 0.05 in each comparison.

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