Figure 1
From: A cell-free assay implicates a role of sphingomyelin and cholesterol in STING phosphorylation
Generation of TBK1-knockout MEFs. (a) Western blots of cell lysates of WT or TBK1-knockout MEFs stimulated with DMXAA for 1 h. (b) Cells were stimulated with DMXAA for 1 h, fixed, permeabilized and stained for endogenous STING, GM130 (a cis-Golgi protein) or TGN38 (a trans-Golgi network protein). Nuclei were stained with DAPI (blue). Scale bars, 10 µm.
