Figure 2

The number of Lyve-1 positive macrophages was inversely correlated with cardiac dysfunction induced by chronic pressure overload. (A–C) Flow cytometry quantification of monocytes (CD45⁺Ly6G⁻CD11b⁺CD64^lo) and L⁻ and L⁺ macrophage subsets isolated from cardiac tissue after 1 week of TAC or SHAM. Data are presented in mean ± SEM. Significance vs SHAM: *P < 0.05, by t test. (D) Quantification by immunofluorescent staining of LYVE-1 positive macrophages identified by CD68 in the heart of SHAM mice and after 1 week of TAC. Data are presented in mean ± SEM. *P < 0.05 by Mann–Whitney test. (E) Flow cytometric analysis of CCR2 in the two main cardiac macrophage subsets after 1 week of TAC (the percent of CCR2 positive L⁻ macrophages was 11 ± 1% in SHAM vs 23 ± 0.8% in TAC in contrast only 1% of L⁺ subsets were positive for CCR2 in hearts submitted to SHAM or TAC). Data are presented in mean ± SEM. Significance ****P < 0.0001 by ANOVA test. ns indicates not significant. (F–H) Flow cytometry quantification of monocytes and L⁻ and L⁺ macrophage subsets in cardiac tissue after 6 weeks of TAC or SHAM. Data are presented in mean ± SEM. Significance vs SHAM: **P < 0.01, by t test. (I) Correlation between the number of L⁺ subsets present in cardiac tissue of each individual mouse after 6 week of TAC and the left ventricular fractional shortening (FS). r_s = 0.72, p = 0.008 by Spearman. The lines come from a linear regression model.