Figure 2

Boring biopsy enables the sampling of a continuous columnar specimen from the surface of the normal brain to the tumor margin and the center of the tumor (measurement marks are defined in millimeters.) (A) Cellular changes and differentiation from normal tissues to the center of the tumor. From the border of the tumor to the center of the tumor, the World Health Organization grade rises from 2 and 3 to 4. Hematoxylin & eosin staining showing dense cellularity, nuclear pleomorphism, and microvascular proliferation at the center of the tumor (B).