Figure 1 | Scientific Reports

Figure 1

From: Optimized assay for transposase-accessible chromatin by sequencing (ATAC-seq) library preparation from adult Drosophila melanogaster neurons

Figure 1

Sample volume and freezing time affect nuclei yield. (a) The number of flies used to collect heads prior to nuclei isolation increases the number of extracted nuclei, but the homogenizer size does not. The indicated volumes of w* Berlin flies were used for nuclei isolation. Data represent the mean ± SD of three independent experiments. (b) Freezing time longer than 5 min decreases GFP detection by flow cytometry. Flies expressing pan-neuronal GFP (nSyb-Gal4 UAS-GFP-nls) were counted at the low gate. Data represent three biological replicates. The data in (a) were analyzed by two-way ANOVA and the data in (b) were analyzed by nonlinear regression using one-phase exponential decay, K = 0.51 min−1, goodness of fit R2 = 0.64. **p < 0.01.

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