Figure 2
Histological analysis of AD mice. (a) Representative H&E-stained (scale bar = 400 μm) cross sections of ears and dorsal skin. (b) Total serum IgE level of FITC-model was measured by enzyme-linked immunosorbent assay (ELISA) (pModel = 0.0127, p7-Met = 0.0159). Each bar represents the mean ± S.E.M. of ten independent experiments. (c) Total serum IgE level of OXZ-model was measured by enzyme-linked immunosorbent assay (ELISA) (pModel = 0.0367, p7-Met = 0.0061). (d) Skin IL-13 level of FITC-model was measured by enzyme-linked immunosorbent assay (ELISA). (e) Skin IL-13 level of OXZ-model was measured by enzyme-linked immunosorbent assay (ELISA). (f) Representative TB-stained (scale bar = 200 μm) cross sections of ears and dorsal skin. (g) The number of mast cells in mouse dorsal skin treated by FITC or 7-Met was measured using ImageJ software. Values are expressed as the means ± S.E.M. (n = 3). (p7-Met = 0.0011). (h) The number of mast cells in mouse dorsal skin treated by OXZ or 7-Met was measured using ImageJ software. Values are expressed as the means ± S.E.M. (n = 3). (pModel = 0.0201, p7-Met = 0.0003). (i) The number of mast cells in mouse ears treated by FITC or 7-Met was measured using ImageJ software. Values are expressed as the means ± S.E.M. (n = 3). (pModel = 0.0108, p7-Met = 0.0019). (j) The number of mast cells in mouse ears treated by OXZ or 7-Met was measured using ImageJ software. Values are expressed as the means ± S.E.M. (n = 3). (pModel = 0.0362) Each bar represents the mean ± S.E.M. of ten independent experiments. #p < 0.05, ##p < 0.01 VS FITC. *p < 0.05, **p < 0.01 VS OXZ. FITC fluorescein isothiocyanate, OXZ oxazolone, 7-Met 7-Methoxyisoflavone, DEX dexamethasone, H&E hematoxylin and eosin, TB toluidine blue.
