Figure 6
7-Methoxyisoflavone downregulated Th17 cells subset. (a) Protein–protein interaction (PPI) network of OXZ-induced differentially expressed (|Foldchange|> 2, q < 0.05) chemokines mRNAs, the nodes in red indicate genes involved in the IL-17 signaling pathway (KEGG: mmu04657, FDR < 0.0001). (b) Relative Cxcl1, Cxcl2 and Cxcl3 mRNA levels were determined by quantitative RT-qPCR. (c) Relative IL-17A mRNA levels were determined by quantitative RT-PCR (p7-met = 0.0096, two-tailed). (d) Immunohistochemistry staining of IL-17A+ Th17 cells. (e) IL-17A positive area ratio was calculated by ImageJ software (n = 6). (pmodel < 0.0001, two-tailed, p7-met = 0.0243). (f) The phosphorylation of MAPKs and STAT3 in the ears total protein was analyzed by Western blotting. (g) The phosphorylation of MAPKs and STAT3 in the dorsal skin total protein was analyzed by Western blotting. (h) The nuclear translocation of AP-1 was analyzed by Western blotting. The expression levels of Ras, c-Raf, p-ERK, ERK, p-JNK, JNK, p-p38 and p38 in the total protein and the nuclear translocation of c-Jun and c-Fos of the right ears were detected by Western blotting. *p < 0.05 and ****p < 0.0001. p-STAT3 phospho-STAT3(Tyr705); STAT3, STAT3(124H6). 7-Met 7-Methoxyisoflavone, p65 (N) nucleus p65, p-ERK phospho-p44/42MAPK(ERK1/2), ERK p44/42MAP Kinase, p-p38 phospho-p38 MAPK (Thr180/Tyr182), p38, p38 MAPK, c-Fos (N) nucleus c-Fos, c-Jun (N) nucleus. In order to avoid the interference of non-specific binding of antibodies to chemiluminescence imaging. Under the premise of ensuring credibility, some WB images are cropped strips, all images have been provided in the Supplementary Document.
