Figure 1
From: TCFL5 deficiency impairs the pachytene to diplotene transition during spermatogenesis in the mouse
TCFL5 distribution during meiosis-I. (A) Immunohistofluorescence of seminiferous tubules stages I–IV, V–VIII and IX–X in TCFL5+/+ mice. TCFL5 (red), SYCP3 (green) and nucleus (Dapi, Blue) were detected. TCFL5 is expressed in spermatocytes but neither spermatogonia nor spermatids. (B) Quantification of TCFL5 expression in spread primary spermatocytes. TCFL5 is mostly expressed from late pachytene to diplotene. (C) Representative immunofluorescence of spread primary spermatocytes in TCFL5+/+ mice. SYCP3 (green), TCFL5 (red) and nucleus (Dapi, Blue) were detected. D) Representative immunofluorescence of spermatids. TCFL5 (red) and nucleus (Dapi, Blue) were detected.
