Figure 4
From: TCFL5 deficiency impairs the pachytene to diplotene transition during spermatogenesis in the mouse
TCFL5 spermatocytes die in the epididymis. (A) TUNEL assay in seminiferous tubules cross-section of TCFL5+/+ and TCFL5−/− mice. Apoptotic cells (red) and nucleus (Dapi, Blue) were detected. TCFL5−/− mice present apoptotic cells in the seminiferous tubules. Yellow arrows show the live multinucleated round giant cells. (B) TUNEL assay in epididymis cross-section of TCFL5+/+ and TCFL5−/− mice. Apoptotic cells (red) and nucleus (Dapi, Blue) were detected. TCFL5−/− mice present apoptotic cells in the epididymis. (C) mRNA expression by qPCR of pro-apoptotic genes Aif and Bax, and anti-apoptotic genes Bcl2, and Hif1a in TCFL5+/+ and TCFL5−/− mice. TCFL5 null mice present alterations in the expression of these genes. (n = 8), *p < 0.05, **p < 0.01, ***p < 0.001.
