Figure 3
From: Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration
Tachyzoites (GFP (green)) transmigrating the EC monolayer (rhodamine-phalloidin (red) and DAPI (blue)) at (a) 12 h and (b) 60 h after infection. The image in (i–iv) at the bottom indicate a cross sectional view of the white box in (a,b). Tachyzoites were (i) either trapped in the EC monolayer or they (ii) migrated into the ECM after transmigration. Another image showing the coincident (iii) transmigration and proliferation (in an EC) of tachyzoites. (iv) Following egression, tachyzoites invade the ECM after disrupting the EC monolayer. Scale bar indicates 50 μm.
