Figure 9
From: Pericytes take up and degrade α-synuclein but succumb to apoptosis under cellular stress
Cell death of primary human brain pericytes in response to pre-treatment with MG132 and α-syn. (a) Representative immunocytochemistry images of MitoSOX staining in primary human brain pericytes after treatment with vehicle, α-syn fibrils (100 nM), MG132 (5 µM) pre-treatment for 6 h following α-syn fibrils (100 nM) incubation and (b) graph showing the integrated intensity of MitoSOX after MG132 (5 µM) pre-treatment for 6 h following α-syn fibrils (100 nM) incubation. Scale bar = 50 µm. (n = 4 epilepsy cases, mean ± SD). *p < 0.05 compared to vehicle, ^p < 0.05 compared to ribbons or fibrils, #p < 0.05 compared to MG132; one way ANOVA with Tukey’s multiple comparisons test. (c) Representative immunocytochemistry images of cleaved-caspase 3 staining in primary human brain pericytes pre-treated with 5 µM MG132 for 6 h prior to incubation vehicle or MG132 and fibrils. Scale bar = 100 µm. (d) Graph showing cleaved-caspase 3 integrated intensity after pericytes were pre-treated with 5 µM MG132 prior to co-incubation with either ribbons (100 nM) or fibrils (100 nM). (n = 4 epilepsy cases, mean ± SD). *p < 0.05 compared to vehicle, #p < 0.05 compared to MG132 treatment, ^p < 0.05 compared to either ribbon or fibrils; one way ANOVA with Tukey’s multiple comparisons test.
