Figure 4
From: BMP2 promotes lung adenocarcinoma metastasis through BMP receptor 2-mediated SMAD1/5 activation

Recombinant BMP2 treatment increases cell migration in human lung cancer cells. (A) The cell migration ability was determined by seeding cells in a migration chamber without Matrigel coating. Cells that migrated through the filter were stained with Liu ‘s solution and counted. Slides were observed under 10 × magnification. Cells were treated with 0, 5, 10 ng/ml recombinant human BMP2 (rhBMP2) in culture medium. (B) Quantitative data derived from (A). Bar graphs represent the average number of migrated cells ± SD. At least three fields were counted for each cell line following rhBMP2 treatment. (C) Representative images of cell migration of each cell line treated with 20 ng/ml rhBMP2 with or without siBMPR2. Slides were observed under 10 × magnification. (D) Quantitative data derived from (C). Bar graphs represent the average number of migrated cells ± SD. At least three fields were counted for each cell line. (E) Representative images of cell migration for each cell line. A549 and H1299 cells were treated with 10 ng/ml rhBMP2 or transfected with siBMP2. Slides were observed under 10 × magnification. (F) Quantitative data derived from (E). Bar graphs represent the average number of migrated cells ± SD. At least three fields were measured for each condition. The p-value was determined by Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001).