Figure 6
From: BMP2 promotes lung adenocarcinoma metastasis through BMP receptor 2-mediated SMAD1/5 activation

Inhibition of BMP2 decreased metastasis in a CL1-5 orthotopic mouse model. (A) A schematic diagram showing the animal experiment. CL1-5 cells stably expressing shLacZ or shBMP2 Luc/GFP were injected into the right lungs of NOD-SCID mice. IVIS analysis was conducted at 7, 14, 28, and 35 days after injection. (B) The body weights of mice were measured three times per week after tumor injection. (C) Metastasis in shLacZ and shBMP2-CL1-5-Luc/GFP groups was measured by IVIS until sacrifice. (D,E) Metastastic lesions in the lungs (D) and nearby organs (E) were analyzed by IVIS after sacrifice. (F) Hematoxylin and eosin (H&E) and IHC stains in mouse right lung tissue for the indicated proteins. Slides were observed under 40 × magnification. Error bars represent SD, and p-value was determined by Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001).