Figure 5 | Scientific Reports

Figure 5

From: Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

Figure 5

Effects of EDNRB overexpression or knockdown on PBC development. (A) Determination of EDN1 and EDN2 mRNAs was accomplished with RT-qPCR technique in sera of PBC mice injected with normal saline (PBC + NaCl), control adenoviruses (PBC + Si Control), Ad-shEDNRB adenoviruses (PBC-EDNRB), and Ad-EDNRB adenoviruses (PBC + EDNRB). Each group contained 10 mice. (B and C) RT-qPCR assay was used to measure levels of EDN1, EDNRA, EDN2, and EDNRB mRNAs in liver tissues of PBC mice injected with normal saline, control adenoviruses, Ad-shEDNRB adenoviruses and Ad-EDNRB adenoviruses. Each group contained 10 mice. (D) Measurement of EDN1 and EDN2 expression at protein levels was examined with western blotting assay in serum samples of PBC mice injected with normal saline, control adenoviruses, Ad-shEDNRB adenoviruses, and Ad-EDNRB adenoviruses. Each group contained 3 mice. (E) Levels of EDN1, EDNRA, EDN2 and EDNRB proteins were determined with western blotting technique in liver tissues of PBC mice injected with normal saline, control adenoviruses, Ad-shEDNRB adenoviruses, and Ad-EDNRB adenoviruses. Each group contained 3 mice. (F) HE staining of liver tissues of mice injected with normal saline, control adenoviruses, Ad-shEDNRB adenoviruses, and Ad-EDNRB adenoviruses. (G–L) Levels of ALP, AST, ALT, AMA-M2, IFN-γ and TNFα in serum samples of PBC mice injected with normal saline, control adenoviruses, Ad-shEDNRB adenoviruses, and Ad-EDNRB adenoviruses were measured with corresponding kits and ELISA assay.

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