Table 3 Overall ranking of best suitable RGs across different species.
Species | Ranking | geNorm | Normfinder | BestKeeper | RefFinder | |
|---|---|---|---|---|---|---|
M value | Stability value | STDEV | coff. of corr | |||
Ladakhi cattle (LAC) | 1 | EEF1A1(0.147) | EEF1A1 (0.171) | RPS15 (0.123) | EEF1A1 (0.978) | EEF1A1 (1.41) |
2 | RPL4(0.147) | RPL4 (0.278) | GAPDH (0.163) | RPS9 (0.973) | RPL4 (2.11) | |
3 | RPS23 (0.186) | UXT (0.297) | B2M (0.391) | RPS23 (0.973) | RPS23 (3.98) | |
4 | RPS9 (0.267) | RPS23 (0.301) | EEF1A1 (0.468) | RPL4 (0.959) | UXT (4.36) | |
5 | UXT (0.322) | RPS9 (0.498) | RPL4 (0.481) | UXT (0.941) | RPS15 (4.76) | |
6 | B2M (0.413) | B2M (0.502) | UXT (0.489) | HPRT1 (0.763) | B2M (5.05) | |
7 | GAPDH (0.512) | GAPDH (0.597) | RPS23 (0.503) | B2M (0.637) | GAPDH (5.12) | |
8 | RPS15 (0.563) | RPS15 (0.602) | RPS9 (0.642) | ACTB (0.433) | RPS9 (5.32) | |
9 | HPRT1 (0.605) | HPRT1 (0.616) | HPRT1 (0.712) | GAPDH (0.017) | HPRT1 (9) | |
10 | ACTB (0.689) | ACTB (0.922) | ACTB (0.812) | RPS15 (0.001) | ACTB (10) | |
Ladakhi Yak (LAY) | 1 | GAPDH (0.223) | GAPDH (0.112) | UXT (0.180) | GAPDH (0.967) | GAPDH (1.41) |
2 | RPS9 (0.223) | RPS9 (0.112) | HPRT1 (0.240) | RPS9 (0.965) | RPS9 (2.21) | |
3 | ACTB (0.386) | ACTB (0.202) | RPL4 (0.500) | EEF1A1 (0.960) | ACTB (3.41) | |
4 | RPS23 (0.507) | RPS23 (0.524) | GAPDH (0.600) | ACTB (0.929) | HPRT1 (3.76) | |
5 | HPRT1 (0.595) | HPRT1 (0.595) | ACTB (0.610) | B2M (0.927) | UXT (3.83) | |
6 | UXT (0.708) | UXT (1.025) | RPS9 (0.680) | RPS23 (0.886) | RPS23 (4.86) | |
7 | B2M (0.892) | EEF1A1 (1.191) | RPS23 (0.840) | RPS15 (0.813) | RPL4 (7.02) | |
8 | EEF1A1 (0.992) | B2M (1.254) | B2M (1.350) | HPRT1 (0.727) | EEF1A1 (7.71) | |
9 | RPL4 (1.121) | RPS15 (1.523) | EEF1A1 (1.590) | UXT (0.001) | B2M (7.74) | |
10 | RPS15 (1.242) | RPL4 (1.538) | RPS15 (1.750) | RPL4 (0.001) | RPS15 (9.74) | |
Ladakhi Donkey (LAD) | 1 | HPRT1(0.250) | HPRT1 (0.123) | ACTB (0.295) | HPRT1 (0.942) | HPRT1 (1.19) |
2 | B2M (0.250) | B2M (0.324) | HPRT1 (0.311) | GAPDH (0.941) | B2M (2.00) | |
3 | RPS23 (0.571) | ACTB (0.518) | RPS23 (0.318) | B2M (0.940) | ACTB (2.45) | |
4 | ACTB (0.612) | RPS23 (0.605) | B2M (0.472) | EEF1A1 (0.927) | RPS23 (3.46) | |
5 | EEF1A1 (0.751) | EEF1A1 (0.851) | RPL4 (0.598) | RPS9 (0.619) | EEF1A1 (5.44) | |
6 | GAPDH (0.857) | UXT (1.181) | UXT (0.613) | RPS15 (0.022) | UXT (6.24) | |
7 | UXT (0.984) | GAPDH (1.208) | EEF1A1 (0.852) | ACTB (0.001) | RPL4 (7.11) | |
8 | RPL4 (1.090) | RPL4 (1.366) | RPS15 (0.92) | RPS23 (0.001) | GAPDH (7.17) | |
9 | RPS15 (1.233) | RPS15 (1.377) | GAPDH (0.95) | UXT (0.00) | RPS15 (8.74) | |
10 | RPSP (1.405) | RPS9 (1.912) | RPS9 (1.635) | RPL4 (0.001) | RPS9 (10.00) | |
Chanthangi Goat (CHG) | 1 | RPS9(0.378) | RPS9 (0.310) | RPS9 (0.1760) | B2M (0.847) | RPS9 (1) |
2 | HPRT1 (0.378) | RPS23 (0.477) | HPRT1 (0.324) | RPS23 (0.676) | HPRT1 (2.38) | |
3 | ACTB (0.434) | ACTB (0.486) | ACTB (0.486) | GAPDH (0.623) | ACTB (2.71) | |
4 | RPS23 (0.636) | HPRT1 (0.740) | RPS23 (0.595) | RPS15 (0.580) | RPS23 (3.13) | |
5 | EEF1A1 (0.803) | GAPDH (0.953) | UXT (0.633) | EEF1A1 (0.435) | UXT (5.48) | |
6 | UXT (0.973) | UXT (1.000) | GAPDH (0.834) | ACTB (0.429) | GAPDH (5.96) | |
7 | GAPDH (1.066) | EEF1A1 (1.120) | EEF1A1 (0.974) | RPS9 (0.422) | EEF1A1 (6.44) | |
8 | RPL4 (1.254) | RPL4 (1.764)) | RPL4 (1.248) | UXT (0.087) | RPL4 (8) | |
9 | RPS15 (1.474) | RPS15 (2.015 | RPS15 (1.869) | RPL4 (0.051) | RPS15 (9) | |
10 | B2M (1.721) | B2M (2.517) | B2M (2.301) | HPRT1 (0.001) | B2M (10) | |
Double hump Camel (DHC) | 1 | B2M (0.600) | HPRT1 (0.295) | HPRT1 (0.203) | GAPDH (0.372) | HPRT1 (1.32) |
2 | RPS9 (0.600) | ACTB (0.418) | EEF1A1 (0.294) | ACTB (0.677) | ACTB (2.63) | |
3 | HPRT1 (0.664) | B2M (0.420) | ACTB (0.377) | RPS9 (0.751) | B2M (2.71) | |
4 | ACTB (0.680) | RPS9 (0.586) | RPS23 (0.462) | EEF1A1 (0.001) | RPS9 (2.99) | |
5 | RPS23 (0.747) | UXT (0.668) | RPS9 (0.542) | RPS15 (0.446) | EEF1A1(4.56) | |
6 | EEF1A1 (0.794) | EEF1A1 (0.731) | B2M (0.572) | RPS23 (0.055) | RPS23 (5.60) | |
7 | UXT (0.860) | RPS23 (0.915) | UXT (0.693) | UXT (0.774) | UXT (5.92) | |
8 | GAPDH (0.992) | RPS15 (1.333) | GAPDH (0.862) | RPL4 (0.599) | GAPDH (8.00) | |
9 | RPL4 (1.180) | RPL4 (1.766) | RPL4 (1.538) | B2M (0.809) | RPL4 (9.00) | |
10 | RPS15 (1.352) | RPS15 (1.856) | RPS15 (1.61) | HPRT1 (0.797) | RPS15 (10.00) | |
Zanskar Horse (ZAP) | 1 | RPS9(0.135) | EEF1A1 (0.631) | UXT (0.341) | B2M (0.947) | RPS9 (1.73) |
2 | RPL4 (0.135) | UXT (0.684) | RPS23 (0.434) | ACTB (0.662) | RPL4 (2.51) | |
3 | RPS23 (0.142) | RPS9 (0.749) | RPS9 (0.465) | HPRT1 (0.447) | UXT (2.66) | |
4 | EEF1A1 (0.194) | RPS23 (0.759) | RPL4 (0.469 | GAPDH (0.257) | EEF1A1 (2.78) | |
5 | UXT (0.249) | RPL4 (0.763) | EEF1A1 (0.498) | RPS15 (0.182) | RPS23 (3.13) | |
6 | RPS15 (0.411) | RPS15 (1.022) | RPS15 (0.816) | EEF1A1 (0.159) | RPS15 (6) | |
7 | HPRT1 (0.824) | ACTB (1.36) | ACTB (1.211) | RPS9 (0.001) | ACTB (7.24) | |
8 | ACTB (1.136) | HPRT1 (1.727) | HPRT1 (1.226) | RPS23 (0.001) | HPRT1 (7.74) | |
9 | B2M (1.394) | B2M (1.945) | B2M (1.914) | UXT (0.001) | B2M (9) | |
10 | GAPDH (1.712) | GAPDH (2.795) | GAPDH (20.99) | RPL4 (0.001) | GAPDH (10) | |
