Figure 1
From: Level of constitutively expressed BMAL1 affects the robustness of circadian oscillations
MYC-BMAL1 expression driven by a DOX-inducible promoter restores rhythmic Bmal1 promoter activity. (A) Schematic diagram of U2OS-PBmal1::Fluc/ΔBmal1/PTRE3Gs::Myc-Bmal1 strains. Endogenous Bmal1 was inactivated by CRISPR-Cas9 and a gene coding MYC-BMAL1 was expressed from the DOX-inducible promoter PTRE3Gs. Bmal1 promoter activity was monitored using the PBmal1::Fluc reporter. We obtained seven strains (-2, -17, -23, -27, -33, -51, and -59). (B) Time course of luminescence measurements from the PBmal1::Fluc reporter in strain-2. Measurements were performed every 20 min and were taken in triplicate. The average values are shown. DOX concentrations are indicated on the right side of the graph.
