Table 4 Primers.

From: Improved β-cell function leads to improved glucose tolerance in a transgenic mouse expressing lipoprotein lipase in adipocytes

Genea

Forward primer

Reverse primer

Snd1

GGG ACG AGA GTA TGG GAT GAT

GCC TTC TGC AAC TAG CGA CT

Ldlr

AGT GGC CCC GAA TCA TTG AC

CTA ACT AAA CAC CAG ACA GAG GC

Lss

TCG TGG GGG ACC CTA TAA AAC

CGT CCT CCG CTT GAT AAT AAG TC

Insig1

TGT CGG TTT ACT GTA TCC CTG T

GTT GAT GCC AAC GAA CAC GG

Lrrc4

GAG GAG CTT GAG ATG TCA GGG

CCA GTC CGT CAA AAG CAT TCC

Ccl19

GGG GTG CTA ATG ATG CGG AA

CCT TAG TGT GGT GAA CAC AAC A

Mcp1

ACA CTG GTT CCT GAC TCC TCT

ATT AGA TTC GGT TTA ATT GGC CC

Il6

CCA AGA GGT GAG TGC TTC CC

CTG TTG TTC AGA CTC TCT CCC T

Ccl19 e1

CAC TCA CTC TCT GTG GCC TG

CAG AGA ACC AGC AGG CTG AA

Ccl19 e2

GAA GAC TGC TGC CTG TCT GT

ACC CTG CAG CCA TCT TCA TT

Ccl19 e3

GTT CAC CAC ACT AAG GGG CT

CTT CGG ATG ATG CGA TCC AC

Ccl19 e4

ATC ACT CTG GCC CAG GAA AC

CTT GGC TGG GTT AGG TCT GG

Ccl19 e1-e4

CAG TCA CTC CCC TGT GAA CC

CTT GGC TGG GTT AGG TCT GG

18S

GTAACCCGTTGAACCCCATT

CCATCCAATCGGTAGTAGCG

  1. aThe sequences of primers used for realtime PCR are indicated. In addition, we designed primers to amplify the four exons of Ccl19 (e1, e2, e3, and e4). The primers used to identify Ccl19 isoforms (CCl19 e1-e4) by PCR in adipose tissue are also indicated.
Back to article page