Figure 5
Autophagy mediated the effect of betulinaldehyde on viability and proliferation of A549 cells. (A) A549 cells were treated with 20-μM betulinaldehyde for 24 h with or without 3-MA before the cell viability was determined via CCK8. (B) The cell proliferation was measured via EdU staining. (C) The statistical analyses of the proliferation ability. (D,E) Colony formation of the A549 cells analyzed via crystal violet staining. All values are expressed as the mean ± SD (n = 3). Comparisons among multiple groups were performed using one-way analysis of variance. The differences between control and other groups were analyzed using LSD multiple comparison test. **P < 0.01 versus control group, #P < 0.05, ##P < 0.01 versus Bet group (Bet = betulinaldehyde, 3-MA = 3-methyladenine). Scale bar, 100 μm.
