Figure 7
Betulinaldehyde inhibited the growth of A549 tumor xenografts in nude mice in vivo. After adaptive feeding for one week, A549 cells were implanted into nude mice via subcutaneous injection under the axilla, and the mice were treated with different concentrations of betulinaldehyde when the tumor grew to a suitable size. (A) The xenograft tumors were separated when the animals were killed. (B) The tumor growth was measured every 4 days. Results were presented as mean ± SD (n = 7). (C) The total body weight was monitored for 21 days. (D–F) The expressions of SQSTM1 and LC3-II were tested at the protein level and the data were quantitated to be represented as mean ± SD (n = 3). Cropped images are displayed, uncropped blots are displayed in Supplementary Fig. 4. The images were collected from different parts of the same gel with same loading amounts. The blot gels shown are representatives of three different experiments (n = 3). Comparisons among multiple groups were performed using one-way analysis of variance. The differences between control and treatment groups were analyzed using LSD multiple comparison test. *P < 0.05, **P < 0.01 versus control group, #P < 0.05, ##P < 0.01 versus Bet group (Bet-L = betulinaldehyde [50 mg/kg]; Bet-M = betulinaldehyde [100 mg/kg]; Bet-H = betulinaldehyde [200 mg/kg].
