Figure 5

Extravascular leakage of FD-10 and expression of Ki-67 in chronic stage of cerebral infarction. FD-10 leakage and the number of Ki-67 cells merged with lycopersicon esculentum lectin (LEL) were quantified in the peri-infarct area shown in (A). FD-10 leakage is also observed in the chronic phase after infarction in wild-type mice (n = 4 each) (B). The relative intensity of FD-10 to the control is shown in the box and whiskers with maximum and minimum (C). This intensity is significantly increased on days 7 and 28. Fluorescence measurements relative to controls. One-way ANOVA followed by Dunnett’s post hoc test for multiple comparisons (C). The extravascular leakage of FD-10 on 7 day (D) is significantly higher in occludin-deficient mice (n = 4) than in wild-type mice (n = 5). The relative intensity in comparison to that in wild-type mice is shown in the box and whiskers with maximum and minimum (E). The yellow squares in (B and D) indicate representative measurement areas. The number of Ki-67 cells merged with LEL per area in the infarcted area was higher in wild mice (n = 4) than in occludin-deficient mice (n = 4) on day 7 after cerebral infarction (F). The arrows indicate that Ki-67 merged into the LEL. Box and whiskers with maximum and minimum are shown (G). Dunnett’s test is performed in (C). Unpaired t-test are performed in (E and G). Scale bar: 100 µm. *P < 0.05. FD-10: fluorescein isothiocyanate-dextran with average molecular weight of 10, 000 Da.