Figure 1

Representative experimental set-up. (a) Placement of NIRS sensor (PortaMon, Artinis Medical Systems, The Netherlands) on the left hindlimb muscle of a Yucatan minipig. (b) The underside of the NIRS sensor consists of one photodiode (light receiving element) and three LEDs (each transmitting light at two wavelengths; ~ 765 nm and ~ 850 nm). The interoptode distances from the photodiode to each LEDs are as follows: 30 mm, 35 mm and 40 mm. The three LEDs emit a total optical power of 0.88 mW, 1.78 mW and 3.67 mW, respectively. (c) Intra-operative placement of spinal cord intraparenchymal (IP) probes to measure local changes in spinal cord PO2 in response to euthanasia. IP probes were placed as part of other NIRS studies. IP probes intraparenchymal probes, PO2 partial pressure of oxygen, NIRS near-infrared spectroscopy.