Figure 2
Effect of diacetylmorphine and KN-93 and KN-92 on CaMKII phosphorylation in cardiomyocytes and a quality control note on proteomics. (A) Protein blot analysis of P-CaMKII (T287) in each group, original blots are presented in Supplementary Information Fig. 1, samples derive from the same experiment and that blots were processed in parallel. Quantification is shown in (B). (C) Peptide ion mass deviation distribution. (D) Peptide ion score distribution. (E) The horizontal coordinate is the isoelectric point of the identified proteins; the main vertical coordinate is the distribution of isoelectric points of proteins. The histogram in the graph corresponds to the number of proteins identified with the corresponding isoelectric points; the secondary vertical coordinate corresponds to the cumulative curve in the graph, indicating the cumulative percentage of proteins with isoelectric points not higher than the corresponding value. (F) The distribution of relative molecular masses of proteins, the horizontal coordinate is the relative molecular weight of the identified proteins; the main vertical coordinate Number of proteins corresponds to the histogram in the figure, indicating the number of proteins with the corresponding relative molecular masses identified; the secondary vertical coordinate corresponds to the cumulative curve in the figure, indicating the cumulative percentage of proteins with no higher than the corresponding relative molecular masses.
