Figure 4
Effects of diacetylmorphine on TPM 1, MYOM 2 in cardiomyocytes. (A) Western blotting of TPM1, MYOM2 in cardiomyocytes of cell, original blots are presented in Supplementary Information Fig. 2, samples derive from the same experiment and that blots were processed in parallel. Quantification is in (B). (C) Western blotting of CaMKIIδ overexpression lentivirus transfection efficiency, original blots are presented in Supplementary Information Fig. 3, samples derive from the same experiment and that blots were processed in parallel. Quantification is in (D). (E) After transfection with CaMKIIδ overexpressing lentivirus, p-CaMKII, TPM1 and MYOM2 expression in cardiomyocytes was detected by western blotting using 100 µmol/L diacetylmorphine intervention, original blots are presented in Supplementary Information Fig. 4, samples derive from the same experiment and that blots were processed in parallel. Quantification in (F–H). All data are expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: P > 0.05.
