Figure 6
From: Sexual dimorphism during integrative endocrine and immune responses to ionizing radiation in mice
Adrenal and blood enriched gene ontology pathways are distinct and dimorphic. Male and female mice were exposed to GCRsim (0 and 50 cGy) and at 14-days post-irradiation blood and adrenal glands were isolated and total RNA sequencing was performed. 50 cGy male and females were compared to 0 cGy sham controls and enriched gene ontology PANTHER analysis converted gene to pathway (Venn Diagrams; shaded orange (female) and green (male) fractions all represent different PANTHER defined biological pathways and the number of genes involved in each pathway) and g:Profiler (dotplot; red denotes molecular function (MF), orange denotes biological pathways (BP), and blue denotes transcription factors (TF), and the number of genes identified in each) for annotated DEG (p < 0.01) in female blood (A) and adrenals (C), and male blood (B) and adrenals (D). Transcription factors analysis of female (orange label) and male (green label) adrenals, p < 0.01 (E). Overlapping blood and adrenal Consensus Pathways Analysis (CPA) for females (orange nodes) and males (green nodes) or both (blue nodes) and representative number of genes within each node. Significant pathways were identified as sharing at minimum one edge, three common genes, and a combined p-value < 0.01 (false discovery rate was not used for selection) (F). Data represent p < 0.01, n = 3 per group. PANTHER analysis orange circles denote biological pathways in female and green circles denote biological pathways in males.
