Figure 3
From: YB1 modulates the DNA damage response in medulloblastoma
YB1 depletion results in differential γH2AX resolution and Chk2 phosphorylation in SHH and Group3 medulloblastoma cells (A and B) MBCs plated for 24 h prior to infection with either control or YB1 overexpressing adenovirus. Following 48 h incubation cells were irradiated with 2Gy and either lysed (immunoblotting) or fixed prior to staining (immunofluorescence). (C) D341 shLuc (control) and shYB1 cells irradiated with 5Gy (Expanded western Supp Fig. 5). (D) ONS-76 shGFP (control) and shYB1 cells irradiated with 10Gy (Additional replicates Supp Fig. 5).
