Figure 4

Ppp4c interacts with exogenous AXIN1 in X. laevis embryo model. (a,b) Co-IP analysis of Ppp4c with AXIN1 and \(\upbeta\)-catenin in X. laevis embryonic AC tissue. AC tissue was dissected at stage 8 and cultured till sibling embryos reached stage 11.5. (c) Schematic structure of Myc-tagged truncated AXIN1 mutants. (d,e) Co-IP analysis of Ppp4c with various AXIN1 mutants in X. laevis whole embryos microinjected with indicated mRNA and harvested at stage 11.5. (a,b,d,e) Embryos were microinjected with indicated reagents (1 ng for each mRNA) at 1-cell stage. n = 50. The membranes were cropped at indicated region specified in Supplementary Information.