Table 1 Bacterial strains and plasmids used in this study.
Strains and plasmids | Functional characteristics | Source |
|---|---|---|
Strains | ||
 H. campaniensis strain XH26 | Wild type | Laboratory stock, deposited at CCTCCM, China |
 E. coli DH5α | Cloning host | Purchased from TaKaRa Bio. Inc. (Dalian, China) |
 E. coli Stab13 | Cloning host | Purchased from Sangon Bio. Inc. (Shanghai, China) |
 Strain XH26/Δhom | Strain XH26 that is hom deficient | This study |
 E. coli DH5α/pUC57-sgRNA | E. coli DH5α containing recombinant plasmid pUC57-sgRNA (2879 bp) | This study |
 E. coli DH5α/pMD19-T-L | E. coli DH5α containing recombinant plasmid pMD19-T-L | This study |
 E. coli DH5α/pMD19-T-R | E. coli DH5α containing recombinant plasmid pMD19-T-R | This study |
 E. coli Stab13/pwtCas9-bacteria | E. coli Stab13 containing plasmid pwtCas9-bacteria | This study |
 E. coli DH5α/pMD19-T-L + R | E. coli DH5α containing recombinant plasmid pMD19-T-L + R | This study |
Plasmids | ||
 pMD19-T | Lac promoter, cloning vector, Ampr | Purchased from TaKaRa Bio Inc (Dalian, China) |
 pUC57 | Lac promoter, cloning vector, Kanr | Purchased from Genscript Bio Inc (Nanjing, China) |
 pwtCas9-bacteria | TetR/tetA promoter, AmpR promoter, bacterial knockout vector, Ampr, Tetr | Purchased from Miaoling Bio Inc (Wuhan, China) |
 pUC57-sgRNA | pUC57 containing the sgRNA | This study |
 pMD19-T-L | pMD19-T containing the left arm of the donor vector | This study |
 pMD19-T-R | pMD19-T containing the right arm of the donor vector | This study |
 pMD19-T-L + R | pMD19-T containing the donor vectors (L + R) | This study |
