Figure 1 | Scientific Reports

Figure 1

From: The fructose-bisphosphate, Aldolase A (ALDOA), facilitates DNA-PKcs and ATM kinase activity to regulate DNA double-strand break repair

Figure 1

ALDOA depletion leads to changes in glycolysis and DNA repair pathways. (A) U2OS cells were transfected with control siRNA or ALDOA siRNA sequences. 96 h following transfection whole cell lysates were prepared and immunoblotted with the indicated antibodies. (B,C) Cells were treated as in a, followed by LC/MS–MS and gene set enrichment analysis (GSEA) was performed with the canonical pathway, or biological process, gene sets in GSEA Molecular Signatures Database. The green curve corresponds to the ES (enrichment score) curve, which is the running sum of the weighted enrichment score obtained from GSEA software, while the normalised enrichment score (NES) and the corresponding P value are reported within each graph (B) ALDOA depletion downregulates glycolysis pathways. (C) ALDOA depletion leads to the downregulation of DNA repair pathways.

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