Figure 1

Generation of Prok2-tTA mice. (a) Targeting strategy for generating Prok2-tTA mice. (b) Coronal brain sections prepared from colchicine-pretreated Prok2-tTA; Actb-tetO-EGFP mice were immunostained for EGFP in brown. tTA-mediated EGFP expression mostly recapitulates reported Prok2 expression in Prok2-tTA mice crossed with Actb-tetO-EGFP reporter mice. Regions containing EGFP + cell bodies are shown in panels with higher magnification. Some stained regions, such as the substantia nigra (SN), contain only EGFP + fibers and few EGFP + cell bodies. Scale bars, 100 µm or 500 µm for high or low magnification images. ac, anterior commissure; AcbSH, nucleus accumbens shell; Arc, arcuate nucleus; Cortex, cerebral cortex; DG, dentate gyrus; fx, fornix; DRN, dorsal raphe nucleus; EW, Edinger-Westphal nucleus; GCL, granule cell layer; GL, glomerular layer; ICj, islands of Calleja; LS, lateral septum; Me, medial amygdala; MPO, medial preoptic area; opt, optic tract; ox, optic chiasm; PAG, periaqueductal gray; Pir, piriform cortex; PPTg, pedunculopontine tegmental nucleus; PVH, paraventricular hypothalamic nucleus; SCN, suprachiasmatic nucleus; SN, substantia nigra; SON, supraoptic nucleus; Str, striatum; VMH, ventromedial hypothalamic nucleus.