Figure 4
Deletion of genes encoding Ni-dependent enzymes counteracts the enhancement of cell growth by Ni supplementation in M9 type C. (a) Illustration showing E. coli [NiFe] hydrogenases. (b) Maximum cell density and (c) maximum specific cell growth rate (μmax) of various E. coli strains in 96-well plates cultured using M9 type C with (blue) or without (green) 0.5 µg/L NiSO4. Error bars indicate the standard deviation. *p < 0.05 (Welch's t-test), n = 8. Figures are original, created using Microsoft PowerPoint 2019 for Windows version 1808 (https://www.microsoft.com/).
