Figure 5

Construction of the IR signatures. (A) Unsupervised clustering of the IR phenotype-related genes in the training cohort to classify patients into two genomic subtypes, termed DEG_cluster 1–2, respectively. IR_cluster, IRscore, SOFA score, mechanical ventilation, Charlson score, ICU, age, and sex were used as patient annotations. Red represents high expression of phenotype-related genes, while blue represents low expression of these genes. (B) Differences in the IRscore between the IR patterns in the training cohort (Mann–Whitney U test). (C) IRscore between the two IR phenotype-related genomic subtypes in the training cohort (Mann–Whitney U test). (D) HFD-45 between patients with high and low IRscores in the training cohort (Mann–Whitney U test). (E–I) Concentrations of D-dimer (E), CRP (F), lactate (G), ferritin (H), and procalcitonin (I) between patients with high and low IRscores in the training cohort (Mann–Whitney U test). (J) IRscore between ICU and non-ICU patients (Mann–Whitney U test). (K) The IRscore of patients with or without mechanical ventilation (Mann–Whitney U test). (L) IRscore of patients with Charlson scores > or ≤ 6 (Mann–Whitney U test). (M) IRscore of patients with SOFA scores > or ≤ 9 (unpaired t test). Asterisks represent the statistical P value (*P < 0.05; **P < 0.01; ***P < 0.001; ns no significance). MV mechanical ventilation.