Figure 1
From: Circ_ZNF778_006 promoted ESCC progression by upregulating HIF-1α expression via sponging miR-18b-5p
The characters and the expression of circ_ZNF778_006 in ESCC tissues. (A) The ___location and the length of circ_ZNF778_006. (B) RT-PCR assay was applied to detect the expression of circ_ZNF778_006 and ZNF778 mRNA treated with RNase R- and R+. ***p < 0.01 versus normal tissues. (C) The expression of ZNF778 mRNA was lower than circ_ZNF778_006 treated with actinomycin D at different times. ***p < 0.01 vs. ZNF778 mRNA. (D) The expression of GaPDH, circ_ZNF778_006 and ZNF778 mRNA in nuclear was lower than that in cytoplasm. And the expression of U6 was reserved. ***p < 0.01 (E) The expression of circ_ZNF778_006 in ESCC tissues (n = 110) was higher than that in normal tissues (n = 110). ***p < 0.01 vs. the normal tissues. (F) The expression of ZNF778 mRNA in ESCC tissues (n = 110) was equal to that in normal tissues (n = 110). (G) The expression of circ_ZNF778_006 in ESCC tissues of stage T1+2 (n = 45) was lower than that in stage T3+4 (n = 65). ***p < 0.01. (H) The expression of circ_ZNF778_006 in ESCC tissues was increased with the Stage increased.***p < 0.01. (I) The expression of circ_ZNF778_006 in ESCC tissues of stage M0 (n = 77) was lower than that in M1 (n = 33). ***p<0.01. J The expression of circ_ZNF778_006 in ESCC cell lines (EC9706) was highest, and the expression was reserved in TE-1 cells. **p < 0.05, ***p < 0.011.
