Figure 2
Source data is available upon request.
Glycan microarrays and mass-spectrometric N-glycan evaluation of native Oo-ASP-1. (a) Glycan microarrays displaying glycan recognition by pooled (n = 11) native antigen-specific antibodies from calves immunised with native Oo-ASP-1 (upper panel) or Pichia pastoris Oo-ASP-1 (lower panel), expressed as Mean Fluorescence Index (MFI). The X-axis contains the structure identification codes for the glycans, as previously published22. Glycan structures are organised based on core fucosylation type, depicted in the top-left corner of each frame. Further modifications are indicated by “A” and “B”, and displayed in the legend below. (b) Mass-spectrometry on PNGase A released N-glycans from native Oo-ASP-1. Differentiation between core α1,3-fucose and core α1,6-fucose was not made in this figure, hence the double displayed N-glycan structures with both core fucose linkage-types. Monoisotopic masses of measured signals are indicated, and the proposed glycan structures are depicted based on the Consortium for Functional Glycomics (CFG) nomenclature. The X-axis displays the mass to charge ratio (m/z), whilst the Y-axis displays the relative intensity in arbitrary units (intens. [a.u.]). Data in (a) are presented as mean values + / − standard error of the mean of four technical replicates. Experiments presented in (a) and (b) were conducted once.
