Figure 2
From: Optogenetic induction of caspase-8 mediated apoptosis by employing Arabidopsis cryptochrome 2
Blue light trigger Opto-Caspase8 cleavage and activation. (A) N-terminal part of cryptochrome interacting basic-helix–loop–helix protein CIB1 (amino acids 1–170, CIB1N) are fused with Caspase8, PHR-Caspase8 is marked as Opto-Casp8 V1 and PHR-Caspase8/CiB1N-Caspase8 as Opto-Casp8 V2. CIB1N, the N-terminal ___domain of CIB1. (B) Co-IP assay showed blue light enhanced the interaction of Opto-Caspase8 V2 in HEK293 T. Transfected HEK293T cells were treated with blue light (30 μmol m−2 s−1) for indicated time before lysed. The immunoprecipitation signals were probed by anti-GFP or anti-Flag, respectively. (C) Blue light actives Opto-Caspase8 V1 in HeLa cells. (D) Cleavage efficiency of caspase8 between Opto-Caspase8 V1 and Opto-Caspase8 V2 in Hela cells.
