Figure 4

Opto-Caspase8 cause cell death in HeLa cells. (A) Detecting the caspase-8 activity with FAM-LETD-FMK with flow cytometry in HeLa cells under blue light, the empty vector was transfected as control. (B) Mean fluorescence intensity (NFI) of (A). Data are presented as mean ± SD (n = 3). Student’s t test: ***p < 0.001. (C) The transfected cells were either shielded or exposed to pulse blue light (50 μmol m⁻² s⁻¹) for 12 h and incubated with DAPI, after washing for 3 times, pictures were obtained by Zeiss Axio Observer A1 microscope, CiB1N-Caspase8 and the empty vector pci myc were co-transfected as control. (D) Relative cell apoptosis rate analysis of (A), rate = number of condensed nuclei in blue/number of condensed nuclei in dark. Data are presented as mean ± SD (n = 3). Student’s t test: ***p < 0.001.