Figure 4

Correlation analysis of fluorescence imaging at different sites of the gastrointestinal tract and ICG injection time. (a) Rats were injected with 1 ml ICG (concentration: 10 mg/ml) through the jugular vein. (b–f) Tissue samples were obtained after ICG injection: 1. Liver; 2. Bile duct; 3. Duodenum; 4. Jejunum; 5. Mid-segment of small intestine; 6. Ileum; 7. Colon. A: Rats Groups A. B: Rats Groups B. C: Rats Groups C. D: Rats Groups D. Tissue samples were collected at 4 h post-ICG injection for Groups A and D, 12 h for Group B, and 20 h for Group D. E: Correlation analysis between the farthest extent of gastrointestinal fluorescence after ICG injection and the time from ICG injection to tissue samples collection.