Figure 2
From: Immediate reuse of patch-clamp pipettes after ultrasonic cleaning
Ultrasonic cleaning allows reusing pipettes for multiple consecutive recordings. (a,b) Change in Rpip before and after cleaning of the tip of low resistance (a) and high resistance (b) pipettes. Single values are represented in grey, while mean ± SEM values are represented in black. (c) Evolution of Rpip over several cleaning stages. (a–c) Ultrasonic cleaning can clean the tip as Rpip is recovered after each cycle. (d) Patch-clamp pipette can be successfully reused at least ten times and RGS is successfully reached. Single values are represented in grey, while mean ± SEM values are represented in black. (e) Ra remains unaffected after each cleaning cycle, indicating that the tip is cleaned and the pipette can be reused for successful patch-clamp recordings. Single values are represented in grey, while mean ± SEM values are represented in black. (f) Ultrasonic cleaning can allow pipette to be reused to reach a gigaseal. S sonification only, S & + P sonification combined with positive pressure only, S & + /− P sonification combined with alternance of positive and negative pressure.
