Figure 3
From: Immediate reuse of patch-clamp pipettes after ultrasonic cleaning
Ultrasonic cleaning is not harmful for neurons. (a) Example of a neuron being recorded before (left), during (middle) and after (right) ultrasonic cleaning of a patch-clamp pipette inside the same recording chamber. Scale bar is y = 20 mV (all panels) and x = 100 ms (left and right) and 20 s (middle). Blue bar corresponds to the moment when the non-recording tip is being cleaned. (b,c) The cleaning procedure does not interfere with simultaneous patch-clamp recordings as RMP and Rm of the recorded neurons remain unaffected. Single values are represented in grey, while mean ± SEM values are represented in black. (d) Example of a neuron being successfully recorded three consecutive times with the patch-clamp pipette at the soma after ultrasonic cleaning. Scale bar is y = 20 mV and x = 100 ms. (e) Changes in Rm from neurons being recorded several times at different cleaning stages. First attempts of recording neurons were performed with a fresh pipette, after four and eight cleaning sequences. (f) Post-hoc staining of a neuron from (d). Scale bar is x = 80 µm.
