Figure 5

Comparison of differentially expressed genes in RAPID-E differentiated cells either with or without TTNPB. (A) Volcano plot analysis of differentially expressed genes in day 6 samples. Red dots are upregulated genes, and blue dots are downregulated. Labelled genes are genes in common with the Limb Development term (GO: 0060173). Differentially expressed genes were analysed at a threshold of > 1log₂ and < -1log₂ fold change and an adjusted p-value of > 0.1. (B) Volcano plot analysis of differentially expressed genes in day 11 samples. Red dots are upregulated genes, and blue dots are downregulated. Labelled genes are genes in common with the Limb Development term (GO: 0060173). Differentially expressed genes were analysed at a threshold of > 1log₂ and < -1log₂ fold change and an adjusted p-value of > 0.1. (C) Heat-map visualising representative gene for lineages key gene markers. Red means upregulation, whereas blue means downregulation. Data presented as relative expression to the mean. Only genes with an adjusted p-value  < 0.1 were considered. (D) Visualisation of the significant genes in day 11 samples matching the Cartilage Development term (GO:0051216). Red bars represent upregulated genes, whereas blue bars represent downregulated genes.