Figure 5

Gene enrichment analysis to identify F2R-related pathways. (A) Screening genes that are positively or negatively correlated with F2R expression to draw a gene heat map. (B) Analysis of 11 genes with significantly different expression related to F2R. (C–D) The enrichment analysis of gene ontology (GO) showed that F2R-related genes were mainly related to the completion of the activation of classical pathways, circulating mediated human immune response, immunoglobulin, cell recognition and phagocytosis. (E,F) The enrichment analysis of the Kyoto Encyclopedia of Genes and Genome (KEGG) showed that F2R-related genes mainly interact with ECM receptor, neuroactive ligand receptor, protein differentiation and uptake, cytokine-cyclin receptor interaction, PI3K-Akt signal pathway, cAMP signal pathway, Wnt signal pathway, cGMP PKG signal pathway and TGF-β Signal path correlation. (G) Gene cluster enrichment analysis (GSEA) showed that the signal pathway related to the expression of F2R mRNA in gastric cancer. The results showed that DNA replication, Huntington’s disease, JAK-STAT signal pathway, MAPK signal pathway and oxidative phosphorylation were related to the expression of F2R. (H) Protein–protein interaction (PPI) network was used to analyze genes related to F2R.