Figure 4
Analysis of the number and morphology of BrdU + /DCX + cells in the C58/J and WT strains according to their distribution within DG regions and layers. (A) A representation of the analyzed regions of interest (rectangles) in the crest, suprapyramidal and infrapyramidal blades in the dorsal DG from C57BL/6 J WT mice is shown (20x magnification. Scale bar: 100 µm). (B) Estimated total number of BrdU + /DCX + cells per mm3 in the whole DG, the crest, suprapyramidal (supra), and infrapyramidal (infra) blades from both strains. Two-way ANOVA followed by Sidak’s correction: DG: F(1,32) = 7.620, *p = 0.0374. (C) Percentage of BrdU + /DCX + cells distributed within the subgranular zone (SGZ) and the granule cell layer (GCL) of the whole DG, the crest, suprapyramidal (supra), and infrapyramidal (infra) blades from both strains. Two-way ANOVA followed by Sidak’s correction: DG-SGZ: F(1, 16) = 8.609, *p = 0.0025; DG-GCL: *p = 0.0025; Crest-SGZ: F(1, 16) = 29.43, *p = 0.0028; Crest-GCL: *p = 0.0028; Supra-SGZ: F(1,16) = 0.08987, p = 0.0512; Supra-GCL: p = 0.0512. (D) Representative examples of BrdU + /DCX + cells categorized as ABC-type, D-type, and EF-type. Percentage of BrdU + /DCX + cells classified according to their morphology within the subgranular zone (SGZ) and the granule cell layer (GCL) of the whole DG (E), the crest (F), suprapyramidal (G), and infrapyramidal (H) blades from both strains. Two-way ANOVA followed by Sidak’s correction: (E) SGZ-ABC: F(1, 24) = 13.35, *p < 0.0001; GCL-EF: F(1, 24) = 12.28, *p = 0.0039; (F) SGZ-ABC: F(1, 24) = 15.32, *p < 0.0001; GCL-EF: F(1, 24) = 9.102, *p = 0.0185; (G) SGZ-ABC: F(1, 24) = 3.721, *p = 0.0351; GCL-EF: F(1, 24) = 7.023, *p = 0.0171; (H) SGZ-ABC: F(1, 24) = 2.385, *p = 0.0125. All results are expressed as mean ± SD. n = 5 animals per group.
