Figure 10
Identification the role of SLC12A2 in CRC cells. (A,B) Colony formation assay (A) and CCK-8 assay (B) in HT29 and LOVO cells transfected with SLC12A2 siRNAs (siSLC12A2 #1, #2) or control siRNAs (siNC). (C,D) Colony formation assay (C) and CCK-8 assay (D) in HCT116 and SW480 cells transfected with pCMV3-SLC12A2 plasmids (SLC12A2 OE) or control empty vectors (Vec). (E) The expression of SLC12A2, c-Myc, Nanog and CD44 were tested by western blotting in HT29 and LOVO cells transfected with SLC12A2 siRNAs (siSLC12A2 #1, #2) or control siRNAs (siNC). (F) The expression of SLC12A2, c-Myc, Nanog and CD44 were tested by western blotting in HCT116 and SW480 cells transfected with pCMV3-SLC12A2 plasmids (SLC12A2 OE) or control empty vectors (Vec). The western blot images in this figure were cropped from full blots and original full blots were presented in Supplementary Fig. S3. Three dependent experiments were performed with similar results. Data shown above were presented as the means ± s.e.m., n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
