Figure 2
From: Mouse auditory cortex sub-fields receive neuronal projections from MGB subdivisions independently
Retrogradely labeled cells were found in MGB. (A) Schematic drawing of tracer injection. According to Optical imaging result, belt areas (UF, AII, and DP) can be identified and 0.1 μL green-CTB and red-CTB were injected into UF and AII separately. (B) Photos of coronal sections with the tracer injection site stained with DAPI under florescence microscope. Scale bar was 0.5 mm. (C) Photos of a DAPI-stained coronal section including the MGB which be merged fluorescence images of retrograde labeling (red and green). The interval distance between two sections is 120 μm as right drawing. (D) Photos of coronal sections stained with SMI-32. Dot line indicates the boundary of each MGB subdivision. (E) Quantitative analyses of the position of retrogradely labeled cells in the MGB. Left: Schematic diagram illustrating the coordinate for quantifying the position of retrogradely labeled cells. A ventrodorsal line passing through the most lateral edge of MGv served as the vertical axis, and a rostrocaudal line passing through the most ventral edge of MGv served as the horizontal axis. Right: Averaged position of labeled region in MGB. Red-points and green-points indicate the center of labeled region by double injection in UF (green) and AII (red) which were calculated by 16 individual animals. Squares reflect labeled cells in MGv and circles reflect labeled cells in MGd; error bars were SD.
