Figure 5
From: Distinct CD16a features on human NK cells observed by flow cytometry correlate with increased ADCC
IgG sensitivity and blocking can differentiate distinct populations in YTS-CD6a cells based on CD16a epitope staining. (A) Representative flow cytometry plots of untreated and kifunensine (Kif.)-treated (20 and 100 µM) YTS-CD16a cells distinctly showing that the 3G8 binding of CD16a on YTS-CD16a cells (x axis) and B73.1 binding of CD16a (y-axis) is affected by IgG blocking and no significant difference is seen comparing both treatment concentrations. Dashed lines indicate the mean fluorescence intensities in each dimension. (B) Representative flow cytometry plots of untreated and kifunensine (Kif.)-treated (20 µM) YTS-CD16a cells gaging sensitivity in differentiating distinct populations based on N-glycan composition at varying percentages of WT and Kif-treated populations.
